Watch: Jimmy Kimmel mocks Prince Harry with spoof book
The story featured on his late night chat show Jimmy Kimmel Live sees a cartoon of Princess Diana appear on a cloud to rub cream on him, in reference to an anecdote in the duke's memoir Spare, in which he reveals he suffered frostbite to his penis during a trip to the North Pole.
Kimmel quipped to his studio audience: "Prince Harold's [book] is so popular, they're now releasing a version for kids. It's a twist on The Princess and the Pea, it's called The Prince and the Penis."
Read more: Inconsistencies found in Prince Harry's book
The comedian read aloud: "At the chilly North Pole, a silly young codger took a walk in the snow, and froze his wee todger."
Kimmel goes on: "'Oh Mummy! Oh Mummy!' He cried with a scream, and from then up on high she appeared with some cream. My poor little prince, put this on your willy. It will lessen the ache and make it less chilly."
A cartoon version of Princess Diana is shown as Kimmel reads: "Mummy knelt down and gave him a squirt, into his trousers where his winkle still hurt."
The story finished: "Back to her cloud his mother went soaring, and said this aloud, 'should you ever have icy-chills on your hard-on, give it a rub with Elizabeth Arden."
In Prince Harry's new autobiography he details how he used the same moisturising cream his mother had, to soothe his "borderline traumatised" penis.
The Duke of Sussex wrote: "I'd been trying some home remedies, including one recommended by a friend, she'd urged me to apply Elizabeth Arden cream.
"'My mum used that on her lips, you want me to put that on my todger?!', 'It works Harry, trust me'."
Princess Diana was killed a car crash in Paris in 1997, when Harry was just 12-years-old.
In his book he admits struggling to process his grief over the loss of his mother.
Spare has become the fastest-selling non-fiction book ever, with 400,000 copies sold across hardback, ebook and audio formats on its first day of publication.